Tth111ii
WebAug 11, 1980 · A second site specific endonuclease with novel specificity has been purified from Thermus thermophilus strain 111 and named Tth111II. The enzyme is active at temperature up to 80 degrees C and requires Mg2+ or Mn2+ for endonuclease activity. Tth111II cleaves phi X174RFDNA into 11 fragments and lambda NA into more than 25 … WebMar 10, 2024 · The best candidate for this role is the endonuclease Tth111II , whose deletion produced a 1,000-fold decrease in transjugation efficiency. This type IIG restriction enzyme has a rather low specific activity and preferentially nicks the top strand at position N11 downstream from its CAARCA recognition sequence.
Tth111ii
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WebTth111II 4 849 1978 1985 2024 UbaJI 12 VspI 2 317 2453 XbaI 1 276 XhoI 1 158 XmnI 2 946 2880 Enzymes that do not cut pET-23a(+): AatII AflII AgeI ApaI ApaBI AscI AvrII BaeI BclI BmgI BseRI BsmI BspMI BsrGI BssHII BstEII BstXI Bsu36I ClaI EcoNI EcoRV FseI HpaI KpnI MluI MunI NarI NcoI NruI NsiI NspV PacI PflMI PmeI PmlI PshAI RleAI RsrII SacII ... WebDec 5, 2011 · The TaqII enzyme is a member of the Thermus sp. enzyme family that we propounded previously within Type IIS restriction endonucleases, containing related thermophilic bifunctional endonucleases-methyltransferases from various Thermus sp.: TaqII, Tth111II, TthHB27I, TspGWI, TspDTI and TsoI. These enzymes show significant …
WebConclusions: TspDTI, TsoI and isoschizomers Tth111II/TthHB27I are closely related bifunctional enzymes. They comprise a tandem arrangement of Type I-like domains, like other Type IIC enzymes (those with a fusion of a REase and MTase domains), e.g. TspGWI, TaqII and MmeI, but their sequences are only remotely similar to these WebAug 11, 1980 · Abstract. A second site specific endonuclease with novel specificity has been purified from Thermus thermophilus strain 111 and named Tth111II.The enzyme is active at temperature up to 80°C and requires Mg 2+ or Mn 2+ for endonuclease activity. Tth111II cleaves φX174RFDNA into 11 fragments and lambda DNA into more than 25 …
Web× Close. The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. WebGenScript List of Common Restriction Enzyme Sites ** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.
WebTth111II is a thermostable Type IIGS restriction enzyme that recognizes DNA sites CAARCA (R 5 A or G) and cleaves downstream at N11/N9. Here, the tth111IIRM gene was cloned and expressed in E. coli, and Tth111II was purified. The purified enzyme contains internally-bound S-adenosylmethionine (SAM).
WebIn silico restriction digest of complete genomes. Choose genome. Find endonucleases yielding fragments within a range: Minimum Maximum. Minimum recognition size for restriction enzymes. Type of restriction enzymes. Only restriction enzymes with known bases (no N,R,Y...) Include Type IIb restriction enzymes (Two cleaves per recognition … phineas and ferb taking care of thingsWebpET-23a DNA 69745-3 His•Tag® sequence. These vectors differ from pET-21a-d (+) by the “plain” T7 promoter instead. pET-23b DNA 69746-3 of the T7lac promoter and by the absence of the lacI gene. Unique sites are shown on the circle. pET-23c DNA 69747-3 map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression ... phineas and ferb take twoWebTth111II is a thermostable Type IIGS restriction enzyme that recognizes DNA sites CAARCA (R = A or G) and cleaves downstream at N11/N9. Here, the tth111IIRM gene was cloned and expressed in E. coli, and Tth111II was purified. The purified enzyme. tso-c55aWebA second site specific endonuclease with a novel specificity has been isolated from Thermus thermophilus strain 111 and named Tth111II. The enzyme is active at … tso c55aWebTth111II is a thermostable Type IIGS restriction enzyme that recognizes DNA sites CAARCA (R = A or G) and cleaves downstream at N11/N9. Here, the tth111IIRM gene was cloned … tso-c58aWebAug 11, 1980 · A second site specific endonuclease with novel specificity has been purified from Thermus thermophilus strain 111 and named Tth111II. The enzyme is active at … phineas and ferb talkingWebAn E. coli strain that carries the Tth111I gene from Thermus thermophilus 111 (T. Oshima). This product is related to the following categories: Restriction Endonucleases T Z, Time … phineas and ferb talking shirt