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Nigericin effect on ph

Webcell volume, that is a shrinking effect at lower Nigericin concentrations and a swelling effect at higher concentrations. Nigericin (0.1-1 nM) significantly decreases cytosolic pH (pHi), and slightly increases the pHi at 5 and 10 nM[2]. Nigericin exhibits higher toxicity on S18 cells than S26 cells, with IC50 of 2.03±0.55 μM and 4.77±2.35 ... WebAcidification of the cytoplasm of Ehrlich ascites carcinoma cells to pH 6.3 arrests DNA synthesis in these cells. Such an effect can be achieved by incubating the cells at pH 6.2 or by adding low concentrations of the K+/H+ antiporter, the antibiotic nigericin, at neutral pH. Glucose and anaerobiosis potentiate the nigericin effect.

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WebAug 2, 2016 · Nigericin (0.1-10 nM) has apparently a dual effect on cell volume, that is a shrinking effect at lower Nigericin concentrations and a swelling effect at higher concentrations. Nigericin (0.1-1 nM) significantly decreases cytosolic pH (pHi), and slightly increases the pHi at 5 and 10 nM. Nigericin exhibits higher toxicity on S18 cells than S26 ... http://www.cnreagent.com/s/sv75647.html csm mazzacorati bologna https://dvbattery.com

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WebJan 3, 2024 · The levels of IL-1β release were close to nigericin, a potent inflammasome activator due to its ability to induce potassium efflux. We found that these activators induced caspase-1 activation and... WebExplain the effects of increasing nigericin concentration on membrane potential ( ΔΨ) and the pH gradient (ΔpH).[6 marks] In a separate experiment, valinomycin was added to the same bacterial cells. The potassium concentration was varied, and the membrane potential (shown as circles) and pH gradient (shown as triangles) were also recorded. WebApr 11, 2024 · For further exploration the important effect of pyroptosis on the pathogenesis of endometritis and intervention role of TFC, LPS combined with nigericin to induce MEECs was used to establish a pyroptosis model of endometritis cells in vitro. Nigericin, a rapid and potent inducer of cell death, enhances the activation of NLRP3 inflammasome, and ... csm mazzacurati

Nigericin - an overview ScienceDirect Topics

Category:K+/H+-antiporter nigericin arrests DNA synthesis in Ehrlich ascites ...

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Nigericin effect on ph

K+/H+-antiporter nigericin arrests DNA synthesis in Ehrlich ascites ...

WebApr 26, 2024 · In three colorectal cancer cell lines (NCI-H747, DLD1, Caco2), growth was optimal near pH 7.4, and the effect of incubation at pH 7.7 varied, with the strongest inhibition of growth in NCI-H747 ... Webfluxes, thereby leading to a large pH decrease in the alkaline zones and a slight pH rise in the acid zones [6]. The opposite pH shifts in different zones near the cell surface, associated changes in membrane permeability, and possible alterations in the cytosolic pH after AP may differentially affect the photoprocesses occurring in chloroplasts.

Nigericin effect on ph

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WebOct 5, 1976 · 1. The effect of ionophores on the intralysosomal pH (as estimated from the distribution of a weak acid or base), on the distribution of 42K+ across the lysosomal membrane, and on the intralysosomal degradation of 125I-labelled bovine serum albumin has been studied. 2. Nigericin and X537A equilibrat … 1. WebJun 21, 2010 · Nigericin is an antibiotic that facilitates an electroneutral exchange of H + - and K +-ions, thereby depleting the ΔpH across the membrane . In contrast, the antibiotic …

WebMay 1, 2024 · Nigericin transports K 1 across biological membranes (in exchange for H 1 ), leading to a decrease in intracellular K 1 concentration and an increased intracellular H 1 concentration (29, 30),... WebJun 25, 2024 · Nigericin antiports K + and H +, an electroneutral exchange, to collapse only the ∆pH. Valinomycin instead carries only larger cations, not protons, and so collapses the ∆ψ while maintaining the ∆pH ( 15 ). BDQ has been proposed to function as a cationic protonophore ( 11 ).

WebDec 14, 2024 · In the light of the nigericin effect on the single-turnover cytochrome-f kinetics and b-phase generation, we further examined the phenotype of anoxic PETC-P171L cells in multiple-turnover conditions. To do so, we repeated the electron transfer ( Figure 4C ) and P700 experiments ( Figure 4D ) in the presence and absence of nigericin. WebA slight increase in the lysosomal pH can be achieved by either Nigericin, a monovalent antiporter, or by CQ, a weak base (20,21).CQ in a concentration of 1 μM was shown to be sufficient to cause ...

WebBy exchanging H + and K + across cell membranes, nigericin shows promising anti-cancer activities in in vitro and in vivo as a single agent or in combination with other anti-cancer drugs through decreasing intracellular pH (pHi).

WebJun 1, 2001 · Therefore, we assessed the effects on pH i of exposing neurons to 5 μM gramicidin D alone or in combination with 10 μM monensin and 1 mM ouabain; in all cases, pH o was 7.35 at 37°C. ... and the addition of 10 μM nigericin failed to further influence pH i. csm margherita di savoiaWebEnter the email address you signed up with and we'll email you a reset link. csm michael starrettWebNigericin has been employed to study anion transport ( Section 2.5) and to modify the pH gradient across energy-conserving membranes. It is often stated that nigericin abolishes … csm messina sud via bottone