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Web20 dec. 2014 · Check operation of the injector without using a sample, under steady conditions, switching the valve from mainpass to bypass (injection volume set to zero) to … WebInformation 4. Sample Pretreatment for HPLC 1) filtration. Attention : If the column is continually connecting in the reverse direction, it may deteriorate. (Case 3) Protein samples that adsorb easily to the column or samples that are slightly soluble in mobile phase Cause : Samples have adsorbed to packing material or deposited in a column. is soda ash the same as soda crystals
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