WebDNA and amino acid sequences for 20 Elimia species, 4 Pleurocera species, 3 Leptoxis species, and 1 Caridina species were retrieved from the NCBI Nucleotide and Protein databases, then aligned using ClustalX2.1. The Caridina species was included as an outgroup to verify that the clustering algorithms were operating properly. After the … WebDec 31, 2013 · The sequences were aligned using the ClustalX2 algorithm (Larkin et al., 2007). DNA constructs. For transfection in eukaryotic cells, the wild type. RFX5 contains an evolutionarily conserved AT-hook. Scanning of the human RFX5 sequence using the Pfam algorithm (Finn et al., 2010), revealed the presence of an AT-hook motif, spanning …
Clustal Omega - fast, accurate, scalable multiple sequence …
WebClustal X (Thompson et al. 1997) is a version of Clustal W with a graphical user interface. The current version is Clustal X2 (Larkin et al. 2007). The program is designed to (1) perform multiple alignments, (2) view the results of the alignment process, and (3) if necessary, improve the alignment. Improving the alignment is facilitated by ... WebJan 22, 2024 · The cDNA sequences were assembled by using DNAMAN software (Lynnon Biosoft), and the deduced amino acid sequences were aligned by using the ClustalX2 algorithm . See SI Materials and Methods for more details. clocks on socks
A Multiparametric Computational Algorithm for Comprehensive …
WebOct 1, 2024 · The amino acid sequences identified by the multiple alignment analysis were visualized with GeneDoc using the ClustalX2 algorithm, ... A phylogenetic tree was constructed by aligning amino acid sequences via the ClustalW algorithm using the neighbour-joining method and 1000 bootstrap replicates, and the results were visualized … WebApr 11, 2024 · The multiple sequence alignment was performed with ClustalX2.0 software and a neighbor-joining tree was constructed by MEGA6.0 software. The conservative sites were predicted by website: ... was used as the internal control gene for gene expression analysis and the data analysis was referred to 2 − Ct algorithm (Livak and Schmittgen. … http://www.clustal.org/clustal2/ bockshornklee hormone