WebThen the UCK1-Luc firefly luciferase activity was measured. ( B ) In HL-60 cells, Flag vector, Flag-USP28-WT or Flag-USP28-CA (C171A), were expressed and treated with CHX (50 μg/ ml) for indicated time intervals. Then the protein levels were analyzed by western blotting (left panel) and statistical analysis of UCK1 was presented (right panel). WebIs it because actin is not sensitive to CHX? I've been doing a cycloheximide chase assay to check the half-life of protein A, and analysis the result using Western Blot.
Cycloheximide (NSC-185) ≥99%(HPLC) Selleck
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Chlorhexidine 7,1% digluconate (CHX) aqueous …
WebCycloheximide is a naturally occurring fungicide produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effects by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome ), thus blocking eukaryotic translational elongation. WebHO-1 decline in the presence of CHX. Western blot analysis confirmed that TRC8 downregulated HO-1 protein expression in a dose-dependent manner (Figure 1b). TRC8 overexpression did WebAug 14, 2024 · Cultures were treated for 2 h with the protein translation inhibitors CHX, blasticidin (Blast), and hygromycin (Hyg), respectively. FRQ and PRD-4 HF were visualized on Western blots with FRQ and FLAG antibodies, respectively. ( C) MMS is an inhibitor of protein expression. Dark-grown cultures (24 h) were exposed to a 2-h light pulse. opening to f9 the fast saga high