Chromomycin-a3
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Chromomycin-a3
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WebChromomycin A3 as a fluorescent probe for flow cytometry of human gynecologic samples Author R H Jensen PMID: 70448 DOI: 10.1177/25.7.70448 Abstract Chemical, physical and optical properties of chromomycin A3 are examined so as to ascertain appropriate staining and analysis procedures for flow cytometry of human gynecologic samples. WebChromomycin A3. 230752-10MG. 80055-168EA 310.68 USD. 80055-168. Chromomycin A3. Chromomycin A3. Antibiotic that inhibits RNA synthesis. Excitation maximum: …
Chromomycin A3 (CMA3) or Toyomycin is an anthraquinone antibiotic glycoside produced by the fermentation of a certain strain of Streptomyces griseus (No. 7). See more In the presence of Mg ions, Chromomycin A3 binds reversibly to DNA, preferentially to contiguous G/C base pairs. When bound to DNA, Chromomycin A3 has a maximum excitation wavelength of 445 See more • in-vitro membrane-impermeant G/C-specific fluorescent DNA-binding dye. • in-vitro antibiotic of gram-positive bacteria, through inhibition of the incorporation of Pi in the RNA. • in-vitro anticancer drug that inhibits RNA synthesis. See more WebChromomycin A3 450 570 B, BV G-C Range 4, 6-Diamidino-2-Phenylindole HCl (DAPI) 372 456 U A-T Range Ethidium Bromide 545 605 G, IG DNA•RNA Hoechst 33258 365 465 U A-T Range Hoechst 33342 355 465 U A-T Range Mithramycin 395 570 BV G-C Range Olivomycin 430 ...
WebChromomycin A 3 is an anthraquinone antibiotic and antitumor agent isolated from S. griseus that is used as a fluorescent probe for DNA with excitation/emission spectra of 445/575 nm. 1, 2 Its DNA binding is specific to two or more contiguous GC base pairs, which makes it suitable for characterizing heterochromatin in plants with species ... WebJan 29, 2024 · CMA3 is an anthraquinone glycosidic antibiotic, produced by Streptomyces griseus ( 21 ). The anti-cancer effects of CMA3 have been proposed since the 1960s ( 22, 23 ). Side effects of CMA3 in advanced breast cancers have interrupted that use ( 24) but it is widely used for DNA staining ( 25 ).
Web其显示的人类染色体带型与Q带或G带相反,异染色质区如染色体的,一般在Q带或G显带中深染, 常染色质 区浅染,而R带则异染色质区一般浅染,常染色质区深染。. 其他R显带的方法包括用chromomycin A3或olivomycin等荧光素染色,或在 细胞周期 的合成期(S期)将 ...
WebDec 4, 2014 · Chromomycin A3 / metabolism Chromomycins / pharmacology HCT116 Cells HL-60 Cells Humans Melanoma / drug therapy* Melanoma / metabolism … hildebrand orthopädieWebApr 6, 2024 · Chromomycin A3 (CMA3, Figure 17.1) is an antibiotic glycoside produced by the fermentation of a certain strain of Streptomyces griseous. In the presence of bivalent … smallwood street redditchWebChromomycin A3 and mithramycin are antibiotics binding preferentially to the GC-rich regions of DNA. They have excitation maxima at 440 nm and emission maxima at 555 and 575 nm, respectively. Unfortunately the low fluorescence intensity … smallwood street baltimoreWeb(Hoechst 33258, Chromomycin A3). Highly purified fractions of chromosomes can then be isolated rapidly from the labeled chromosomes using the MoFlo High performance Cell Sorter. Chromosome preparation and staining. Block cells at metaphase using 0.1 µg/ml demecolcine for an optimized amount of time to maximize the percentage of mitotic cells smallwood store floridaWebChromomycin A3, is used as a G-C specific DNA ligand which inhibits transcription and acts as a DNA binding dye. Chromomycin A3 antagonizes enhanced DNA binding of … hildebrand orthopädie halleWebBiosynthesis of mithramycin, chromomycin A3, and their derivatives has been investigated in detail . In addition to their antiproliferative potency, AA antibiotics demonstrate an antiviral activity [9,10]. Mithramycin has been tested as a neuroprotector for Huntington’s disease and neuronal death [11,12]. smallwood street mosqueWebThis article is cited by 34 publications. Sangita Majee,, Ranjan Sen,, Suranjana Guha,, Dhananjay Bhattacharyya, and, Dipak Dasgupta. Differential Interactions of the Mg2+ Complexes of Chromomycin A3 and Mithramycin with Poly(dG-dC)·Poly(dC-dG) and Poly(dG)·Poly(dC). smallwood street cumberland md