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7.24: Calculating pH of Buffer Solutions- Henderson-Hasselbalch ...

Web4. Add 50 µl of CB Reaction Buffer to each sample. 5. Add 2 µl of the 10 mM CB Substrate Ac-RR-AFC (200 µM final concentration). Note: For negative control, add 2 µl of CB Inhibitor (Optional). 6. Incubate at 37°C for 1-2 hour. 7. Read samples in a fluorometer equipped with a 400-nm excitation filter and 505-nm emission filter. steelers xbox background

Piping Diagram - Cemline

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